水泡性口炎病毒对IPEC-J2细胞因子转录时相的影响

Transcriptional profiles of intestinal pig epithelial cell jejenum 2 cultures infected by vesicular stomatitis virus

摘要:

摘要：【目的】为了更好的了解水泡性口炎病毒（VSV）感染后引起细胞炎性反应特别是炎性细胞因子的反应，探讨宿主-病毒之间的作用关系。【方法】我们运用荧光定量PCR技术，测定和分析VSV感染IPEC-J2细胞引起的病毒RNA量的变化和细胞因子IL-2、6、8、10、12、IFN-α、 IFN -γ、TNF-α、TGF-β和TLR3分泌水平。【结果】我们发现，VSV感染后引起IPEC-J2细胞分泌IL-6、8、12、TNF-α和TLR3显著增加，TGF-β无显著差异；IPEC-J2细胞不分泌IL-2、IL-10、IFN-α和IFN –γ。【结论】水泡性口炎病毒感染可引起IPEC-J2细胞炎性分子分泌增加。

Abstract:

Abstract: [Objective] To better understand the host inflammatory responses, in particular inflammatory cytokines responses of Vesicular Stomatitis virus (VSV) infection, and the host-VSV interaction. [Methods] We used VSV Indian strain to infect the Intestinal Pig Epithelial Cell Jejenum (IPEC-J2) cell. Then we measured and analyzed the viral RNA by using real-time PCR. The transcript level of cytokines (IL-2, 6, 8, 10,12, IFN-α, IFN –γ, TNF-α, TGF-β and TLR3) were detected by real-time PCR. [Results] We found that the transcript levels of IL-6, IL-8, IL-12, TNF-α and TLR3 were increased obviously whereas TGF-β showed no significant difference. TPEC-J2 cell did not secrete IL-2, IL-10, IFN-α and IFN–γ. [Conclusion] The level of inflammatory response was increased when VSV infected TPEC-J2 cell.