温度对厚指海绵Pachychalina sp.体内真细菌的影响

Effect of temperature on the eubacteria associated with marine sponge Pachychalina sp

摘要:

摘要：温度是影响微生物多样性的重要因素之一。【方法】本研究采用16S rDNAs扩增产物酶切片段多态性（amplified rDNA restriction analysis , ARDRA）和16S rDNAs序列分析方法，对生长在16℃和30℃条件下厚指海绵Pachychalina sp.体内真细菌（eubacteria）的多样性进行了研究，【目的】探讨温度对海绵体内细菌的影响。【结果】根据ARDRA 聚类分析，16℃条件下海绵体内100个真细菌的16S rDNAs克隆片断被分成34个类群，而30℃条件下，海绵体内100个细菌的16S rDNAs克隆片断则被分为32个类群，说明在不同温度条件下，海绵体内真细菌的组成与群落结构有所变化，但研究发现温度没有明显改变海绵体内真细菌总的群落结构。【结果】根据厚指海绵Pachychalina sp.体内真细菌的16S rDNAs序列分析结果发现：在16℃和30℃条件下，海绵体内的真细菌均属于α、γ、δ-变形菌、硫细菌、硫还原菌和烃类分解菌，此外还有少数放线菌。16℃条件下海绵体内的优势菌为γ-变形菌，而且体内的硫细菌和硫还原菌主要是耐寒细菌，而30℃条件下海绵体内的优势菌为α-变形菌。该研究还发现：同一ARDRA类型的克隆序列分析表明在同一ARDRA群内各组分间彼此关系比较密切。

Abstract:

Abstract: Temperature is important factor affecting the bacterial diversity. [Objective] In order to disclose the effect of temperature on the diversity of microorganisms, [Methods] The diversity of microorganisms associated with the sponge Pachychilina sp. at 16℃ and 30℃ of the seawater in the Gulf of Zhanjiang was examined by a restriction fragment length analysis termed ARDRA (amplified rDNA restriction analysis) and 16S rRNA-encoding DNA (rDNA) sequence analysis The computer-aided clustering was performed after separate restriction analysis with enzymes HaeⅢ. [Results] By this method, 100 cloned 16S rDNA fragments were clustered into a total of 34 different groups at 16℃ and 32 different groups at 30℃. HaeⅢ ARDRA patterns showed different among eubacteria living at different temperature degree, whereas the whole communities of eubacteria were not changed distinctly. Screening 60 clones by sequence analysis suggested vast majority of eubacteria related to α, γ, δ subclasses of the class Proteobacteria, some related to sulfur bacteria, desulfobacter, and hydrocarbon utilizing bacteria, minority belonging to actinomyceten at 16℃ and 30℃. α subclass of the class Proteobacteria were the predominant bacteria at 30℃, and γ-Proteobacteria were predominant bacteria at 16℃, additionally sulfur bacteria and desulfobacter were mainly attributed to chilly-enduring bacteria. Sequence analysis of clones with an identical ARDRA pattern confirmed that members of an ARDRA group were closely related to each other.