腾冲热海七株高温厌氧菌的分离及鉴定

Isolation and identification of seven thermophilic and anaerobic bacteria from Hot Springs in Tengchong Rehai

摘要:

摘要：【目的】了解中国云南腾冲热海环境中厌氧菌的分类学特征及生理生化特性。【方法】利用Hungate厌氧操作技术，从云南腾冲热海80-93℃温泉中分离出7株高温厌氧菌，对其进行形态、生长特征及16S rRNA基因序列分析，确定菌株的系统发育地位。【结果】7株菌株细胞形态均为杆状，不产芽孢，革兰氏阴性，严格厌氧，在70℃生长良好。其中较典型的菌株RH0802能在55-80℃温度范围内生长，最适生长温度为70℃；生长pH值范围为5.5-8.5，最适pH值为7.0，能利用葡萄糖、淀粉、甘露醇、甘露糖、核糖、麦芽糖、纤维二糖、木糖、果糖、半乳糖、木聚糖、甘油，不能利用蔗糖、丙酮酸。16S rRNA基因序列相似性的比较分析表明，其中5株菌与Caldanaerobacter属菌株的最高相似性均在98%以上，而RH0804与RH0806分别为96%和93%。菌株RH0802-RH0808的序列登录号分别为FJ748766、FJ748762、FJ748761、FJ748763、FJ748765、FJ748764和FJ748767，在中国普通微生物菌种保藏中心的保藏号分别为CGMCC1.5134-1.5140。【结论】分离自腾冲热海的7株嗜热厌氧菌与Caldanaerobacter属菌株有较高相似性，可将这7株菌株鉴定为Caldanaerobacter属菌株（Caldanaerobacter. sp），其中菌株RH0804和RH0806有成为新种的可能。

Abstract:

Abstract: [Objective] In order to study the taxonomic characteristic and physiological, biochemical properties of anaerobic bacteria from hot springs in Tengchong Rehai, Yunnan Province, China. [Methods] Using Hungate anaerobic technique We isolated seven strains from hot springs in Tengchong Rehai, Yunnan province, and analyzed their 16S rRNA gene sequences. [Results] The seven isolates were rod-shaped, Gram-negative, obligate anaerobe, and spores formation was not observed. All strains could grow well at 70℃. Growth of strain RH0802 occurred between 60 and 80℃, optimally around 70℃. The pH range for its growth was between 5.5 and 8.5, with an optimum around 7.0. Strain RH0802 grew on a wide range of carbon sources, including glucose, starch, mannitol, mannose, ribose, maltose, cellobiose, xylose, fructose, galactose, xylan and glycerol, but it could not utilize sucrose or pyruvate. 16S rRNA gene phylogenetic analysis showed that the maximum similarity between the five strains and the strains of genus Caldanaerobacter was up to 98%, except RH0804 and RH0806, which reached to 96% and 93%,respectively. The two isolates were presumed to be potential novel species. The GenBank accession numbers of RH0802 to RH0808 were FJ748766, FJ748762, FJ748761, FJ748763, FJ748765, FJ748764 and FJ748767. [Conclusion] The results showed that the seven thermophilic anaerobes belonged to the genus Caldanaerobacter.