三种动物粪便及一种虫体可培养放线菌的多样性及其生物活性

Diversity and bioactivities of cultured actinomycete isolated from animal feces and Xylocopa dissimilis

摘要:

摘要：【目的】分析大熊猫、斑马和竹鼠粪便及竹虫的可培养放线菌多样性及其生物活性,探索其作为资源开发利用的可能性。【方法】采集大熊猫、斑马和竹鼠的新鲜粪便及竹虫幼虫，用4种培养基分离放线菌。测定代表菌株的16S rRNA基因序列，并进行系统发育分析，将菌株鉴定到属。测定了分离菌株的水解活性和多种酶活。【结果】共获得121株放线菌，选取的47株代表菌株经系统发育分析，被鉴定为放线菌的9个属。从竹鼠粪便分离到6个已知稀有放线菌属菌株。从其它动物粪便中共分离到4个可能新种。121株菌的水解活性和酶活性不仅高且多样。部分菌株能很好的降解鸡毛和纤维素。【结论】动物粪便放线菌不仅对动物食物的消化吸收有重要作用，也可作为探索工业产品包括酶制剂和生物活性物质的开发资源。

Abstract:

Abstract: [Objective] To study the diversity and bioactivities of cultured actinomycete from feces of Ailuropoda melanoleuca, Equus burchelli and Rhizomys sinensis, and Xylocopa dissimilis body. [Methods] Fresh feces samples of animals’ and Xylocopa dissimilis were collected. We used four media to isolate the actinomycete. Strains were identified by phylogenetic analysis of 16S rRNA gene sequences. Hydrolization and enzyme activities of isolates were examined. [Results] Actinomycetes (121) from the samples were isolated on four media. Among them 47 strains belonged to 9 actinomycetes genera. Six known rare genera and one possible new genus of actinomyctes from Rhizomys sinensis were identified. Three possible new species from other three samples were identified. Some strains could hydrolyze chicken feather and cellulose. [Conclusion] It is considered that the coprophilous microorganisms not only have important function on the digestion and absorption of animal feed, but also are important resource for development of industrial products, including enzyme and bioactive metabolites.