一株蛹拟青霉(Paecilomyces militaris )深层发酵产物中抗肿瘤活性物质分析

Anti-tumor compounds from submerger fermentation products of a Paecilomyces militaris strain

摘要:

摘要： 【目的】初步搞清一株蛹拟青霉（Paecilomyces militaris ）菌株RCEF0927在发酵罐发酵条件下发酵液的抗中国仓鼠卵巢瘤（CHO）细胞活性及活性成分的具体组成。【方法】用刃天青（Resazurin）法测定样品对CHO细胞的抑制率；用高效液相色谱-高分辨质谱和活性测定联用的方法进行活性成分分析和鉴定。【结果】活性测定结果表明菌株RCEF0927经发酵罐发酵的发酵液具有较强的抗CHO细胞活性；提取实验结果表明抗肿瘤活性物质能较好地被乙酸乙酯提取出来；液相色谱-质谱-活性测定分析表明

Abstract:

Abstract: [Objective] The aim was to identify the anti-tumor compounds of the submerger fermentation broth of Paecilomyces militaris (strain RCEF0927). [Methods] Anti-tumor activity was tested with a resazurin cytotoxicity assay model with Chinese hamster ovary (CHO) cells. Bioactive compounds were analysed with combined method of High Performance Liquid Chromatography (HPLC), High Resolution Mass Spectrometer (HRMS) and anti-tumor assay. [Results] Activity test showed that the submerger fermentation broth had strong cytotoxicity against CHO cells. Extraction tests showed that ethylacetate was the best solvent for the bioactive constituents extracting. HPLC-DAD- HRMS-Cytotoxicity assay revealed that the molecular formulars of the active compounds in the extract were possibly C10H13N5O3, C22H22O9, C23H24O10, C22H22O10, C15H10O4, C15H10O, C36H70O11, C36H68O12, C38H74O11, C40H76O13 and C44H82O14. [Conclusion]The possible structures of the bioactibe compounds were deduced with bioassay, HRMS, Uv and database inquiry as cordycepin, daizein, genistein 7,4′-dihydroxyisoflavone-7-O-(4″-O-methyl)-?-D-glucopyranoside, 7,4′-dihydroxy-6- methoxyisoflavone-7-O-(4″-O-methyl)-?-D-glucopyranoside, 5,7,4′-trihydroxy- isoflavone-7-O-(4″- O-methyl)- ?-D- glucopyranoside, polyoxyethylene ether oleate, dehydro products of polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxypropylene or polyoxybutene contained derivants of polyoxyethylene sorbitan fatty acid ester. Cytotoxicity of the compounds was revealed for the first time.