一株金黄色葡萄球菌小菌落突变株的分离鉴定

Isolation and identification of a Staphylococcus aureus small colony variants

摘要:

摘要：【目的】由于金黄色葡萄球菌（金葡菌）小菌落突变株（small colony variants，简称SCVs ）可引起持续复发性感染，且对氨基糖苷类有抗药性，在临床诊断和治疗上造成很大的困扰。我国国内尚无金葡菌SCVs的报道，本研究旨在分离鉴定出金葡菌SCVs菌株，为国内进行SCVs的相关研究提供生物学材料。【方法】通过细菌的形态鉴定、种特异性基因（nuc）的PCR扩增鉴定以及系列生化实验，从人源、动物源及环境源共104株金葡菌分离株中筛选得到金葡菌SCVs，并通过甲萘醌、硫胺素、胸腺嘧啶和血红素等补

Abstract:

Abstract: [Objective] Staphylococcus aureus small colony variants (S. aureus SCVs) could lead to persistent, recurrent infection with the characteristics of aminoglycosides antibiotics resistance, making them a big challenge for clinical diagnosis and therapy. We aimed at isolating and identifying isolates of S. aureus SCVs and providing the biological material of SCVs study in China. [Methods] The combination assays of observing colony phenotype, identification of the species-specific gene nuc of S. aureus by PCR amplification and a series of biochemical tests were conducted on 104 clinical isolates originally isolated from human, cow and environment. The suspected isolates were confirmed as S. aureus SCVs by complementation assay with supplementation of menadione, thiamine, thymine and haemin. [Results] One of the isolates from environment was identified as SCVs, named CDC54 with the species-specific gene nuc of Staphylococcus aureus (S. aureus) confirmed by PCR amplification, whose major phenotypes included smaller colony, decreased pigmentation, decreased coagulase, reduced fermentation of lactose, decreased haemolytic activity, increased resistance to aminoglycosides.[Conclusion] The CDC54 will play an important role in studying prevention, control and pathogenesis for S. aureus SCVs infection.