超高压处理对副溶血性弧菌的影响研究

Effect of ultra high pressure treatment on Vibrio Parahaemolyticus

摘要:

摘要：【目的】探讨超高压致死微生物的机理。【方法】本文以副溶血性弧菌为对象，研究了超高压处理对副溶血性弧菌的灭菌效果、对副溶血性弧菌细胞超微结构、细胞无机盐离子含量以及细胞膜蛋白的影响。【结果】结果表明，在20℃下分别经100、200 MPa高压处理10min后，副溶血性弧菌致死率为40%、84.7%，经300 MPa及以上的压力处理，副溶血性弧菌的致死率为100%。超高压处理对细菌细胞形态结构造成明显的损伤：局部细胞壁遭到破坏，出现缺口；胞质内含物结构紊乱，出现泄漏，细胞中部出现透电子区；细胞结构不完整

关键词:关键词：超高压处理;副溶血性弧菌;细胞膜损伤

Abstract:

Abstract: [Objective] This study explored the mechanism of the microbial inactivation by high hydrostatic pressure . [Methods] We investigated the damages inflicted on the Vibrio Parahaemolyticus cells treated by high hydrostatic Pressure. The effect of ultra high pressure treatment on inactivation of Vibrio Parahaemolyticus cells was studied, the damages inflicted on the microstructure and the membrane proteins of the Vibrio Parahaemolyticus cells were also analyzed.[Results]The result indicated that after pressure treatment at 100 MPa,200 MPa and 20℃ for 10 min, the viable cell was reduced to 60 % and 15.3 % respectively, viable cell counts were not detected following pressure treatment at 300 MPa and 20℃ for 10 min.High pressure treatment at 300 MPa also caused changes in the microstructure of the cell, such as the cell wall of Vibrio Parahaemolyticus was shrinked, breached and leaked out cytoplasm ,the layer structure of cytoplasm disappeared ,and a large electron transmission area appeared in the cell after compression, the membrane disappeared ,the nucleoids condensed and the proteins aggregated after compression. At the same time, the membrane proteins of cells were damaged, and the cell membrane Permeability increased, resulting in leakage of the cell. [Conclusion]This study showed that the damaged cell membrane was the major reason of the mechanism of microbial inactivation by high hydrostatic pressure.

Key words:Keywords: ultra-high pressure treatment; vibrio parahaemolyticus; the cell membrane damage