一株菊酯类农药降解菌的分离鉴定及其降解酶基因的克隆

Isolation, identification and degrading gene cloning of a pyrethroids- degrading bacterium

摘要:

摘要：【目的】筛选分离高效降解菊酯类农药的光合细菌，研究其降解特性，并对该菌株中降解酶基因进行克隆与初步分析。【方法】根据分离菌株的细胞形态结构、活细胞光吸收特征、生理生化特征及其16S rDNA序列系统发育分析鉴定降解菌，气相色谱法测定该菌株降解菊酯类农药的能力，PCR方法克隆降解酶基因。【结果】菌株PSB07-21属红假单胞菌属（Rhodopseudomonas sp.），其降解最佳条件为3000 lx、35℃、pH 7，在此条件下培养15 d对600 mg/L甲氰菊酯、氯氰菊酯、联苯菊酯降解率分别为

Abstract:

Abstract: [Objective] This study aimed to isolate, identify and clone degrading gene of a synthetic pyrethroids degrading bacterium. [Methods] A photosynthetic bacterial strain PSB07-21 capable of degrading several synthetic pyrethroids efficiently was isolated by an enrichment culture. PSB07-21 was identified based on its morphology, physiology and phylogenetic analysis of 16S rDNA sequence. The degradation ability of this strain was evaluated with gas chromatography.The degrading gene was cloned with PCR. [Results] PSB07-21 was closely related to Rhodopseudomonas sp. The optimum condition of degrading synthetic pyrethroidss was at 35℃, pH 7 and 3000lx. PSB07-21 could degrade fenpropathrin, cypermethrin and bipthenthrin by 66.63%, 43.25% and 50.18% in a concentration of 600 mg/L at day 15, respectively. We cloned a putative gene which was 326bp long with 37.0% identical to 2OG-Fe(II) oxygenase gene. When compensating low concentration Fe(II) in PSB medium with synthetic pyrethroids, the degradation efficiency of PSB07-21 was enhanced.[Conclusion] The strain has the potential application to synthetic pyrethroids bioremediation.