超嗜热古菌遗传操作系统研究进展

Advance in Genetic Manipulation Systems of Hyperthermophilic Archaea-A review

摘要:

摘要：遗传操作系统，是研究基因和基因产物功能的一个极为重要的工具。超嗜热古菌遗传操作系统方面的研究落后于甲烷菌及嗜盐古菌中的研究，主要原因是选择标记的缺乏。然而，近十年来，在以硫化叶菌(Sulfolobus)为代表的超嗜热泉古菌和Thermococcus kodakaraensis为代表的超嗜热广古菌中，遗传操作系统研究取得了很大的进展。本文主要对这两种超嗜热古菌的遗传操作系统进展以及应用进行概述。

Abstract:

bstract: Genetic manipulation systems play an important role in the functional analysis of genes and gene products. The development of genetic system in hyperthermophilic archaea is lagging behind those in methanogenic and halophilic archaea. The main problem is the unavailability of efficient selective markers. However, in the past 10 years, researchers have made great progress in the development of genetic systems in the genus Sulfolobus and Thermococcus kodakaraensis, representatives of hyperthermophilic archaea. Here we summarize the latest progress in genetic systems and their application for hyperthermophilic archaea.