先锋牧草-香根草联合固氮菌多样性研究

Diversity of associated nitrogen-fixing bacteria isolated from the pioneer plants-Vetiver zizanioides

摘要:

摘要：【目的】香根草（Vetiver zizanioides）是一种多年生禾本科草本植物，具有极强的生态适应性和抗逆能力，可作饲料和水土保持用。通过研究香根草联合固氮菌多样性，为进一步研究和应用打下基础。【方法】采用无氮培养基，首次从香根草中分离到47株联合固氮菌，分别应用SDS-PAGE全细胞蛋白质电泳、DNA指纹图谱、唯一碳源和16S rDNA全序列测定等方法，进行聚类和多样性分析。【结果】SDS-PAGE、IS-PCR和Bio-BIQA碳源利用的聚类结果基本一致，将供试菌株分为6个类群和4个单菌株；

Abstract:

Abstract: [Objective] Vetiver zizanioides is a perennial grass of the Poaceae family, known as silage, soil and water conservation role. The aim is to collect and identify the resources of the nitrogen-fixing bacteria associated with Vetiver zizanioides. [Methods] Associated nitrogen-fixing bacteria isolated from Vetiver zizanioides, were studied by SDS-PAGE whole-cell protein patterns, insert sequence (IS)-PCR finger printing, utilization of sole carbon sources and 16S rRNA gene sequence analysis. [Results] Based on the results of finger printing analysis, protein patterns and biological test, isolates were grouped into 6 clusters, except 4 single strains. Phylogenetic analysis of 16S rDNA sequences indicated that isolates belonged to Herbaspirillum frisingense, Enterobacter ludwigii, Pseudacidovorax intermedius, Mitsuaria chitosanitabida, Pseudomonas putida, Pseudomonas fluorescens, Burkholderia vietnamiensis and Enterobacter cloacae. [Conclusion] The nitrogen fixers associated with Vetiver zizanioides showed great diversity and may have a potential application for the grass forage and agriculture.