电融合方法建立抗结核杆菌单克隆抗体细胞株
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    摘要:

    By using the electrofusion tecndque, the fusion of NSI myeloma cells with spleen cells of BALB / c mouse immunized with five species of mycobacteria was caaced cuL With the ophmal electrofUSion condihons, we obtained a high fuSion efficiency of l.75 hybrid cell clones per 105 spleen cells. ELISA was used for screening secrehng anhmycobacteria McAb hybrid cells. After cloning, 22 stable hybrid cells'lines were selected, and 8 of them had been studied.

    Abstract:

    By using the electrofusion tecndque, the fusion of NSI myeloma cells with spleen cells of BALB / c mouse immunized with five species of mycobacteria was caaced cuL With the ophmal electrofUSion condihons, we obtained a high fuSion efficiency of l.75 hybrid cell clones per 105 spleen cells. ELISA was used for screening secrehng anhmycobacteria McAb hybrid cells. After cloning, 22 stable hybrid cells'lines were selected, and 8 of them had been studied.

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郭明霞. 电融合方法建立抗结核杆菌单克隆抗体细胞株. 微生物学报, 1998, 38(5):

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