链霉菌ZG0429的分类鉴定与链霉亲和素的分离纯化研究

Research on the classification of Streptomyces strain ZG0429 and purification of streptavidin

摘要:

从土壤中筛选得到1株高产链霉亲和素的放线菌ZG0429，根据形态观察、培养特征、生理生化鉴定以及16S rRNA序列分析，初步判定该菌株为链霉菌属中的淡紫灰链霉菌（Streptomyces lavendulae）。经发酵，ZG0429的链霉亲和素产量可达201.0mg/L。进一步采用硫铵沉淀和凝胶过滤层析纯化，链霉亲和素的回收率为76.87％，纯度可以达到97.03％。该方法简单易行，成本低廉，可得到高产量、高纯度、高活性的目的蛋白，为链霉亲和素发酵产品的大规模纯化提供了依据。

Abstract:

Actinomycete strain ZG0429 with high production of streptavidin was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain ZG0429 was placed within the genus Streptomyces and identified as the species Streptomyces lavendulae. The production reached 201.0mg/L under our fermentation conditions. With the effective system of ammonium_sulfate deposition and gel filtration chromatography, streptavidin was successfully purified from the culture supernatant with an overall recovery of 76.87% and purity of 97.03％. This study illustrates an effective and inexpensive method to produce the aimed protein with favorable yield, high purity and desirable activity, so it provides a valuable reference for the large_scale industrial production and isolation of streptavidin.