山羊传染性胸膜肺炎病原体4株国内分离株的重新分类

Reclassification of the four china isolated strains of the pathogen for contagious caprine pleuropneumonia

摘要:

山羊传染性胸膜肺炎(Contagious Caprine Pleuropneumonia,CCPP)是由山羊支原体山羊肺炎亚种(M.capricolum subsp.capripneumoniae,Mccp)引起的高度接触性传染病,对4株CCPP中国分离株进行分子特征研究,确定其分类地位。针对3段基因(A、B、C),对扩增产物进行酶切鉴定和测序,将结果与丝状支原体簇的6个成员进行遗传衍化分析。在A片段,4株中国分离株的扩增产物经PstI酶切后的结果与Mccp代表株F38相同,为548、420、128等3条带,其他5个丝状支原体簇成员只有420、128bp两条带。在B片段,序列分析结果显示4株中国分离株与F38同源性为99.5%,与山羊支原体山羊亚种Mcc代表株kid的同源性为98.9%,与丝状支原体山羊亚种MmcZZ株同源性仅为95.4%。在C片段研究发现,4株中国分离株的序列与Mmc模式株PG3株同源性为67.4%~67.6%,与2株Mcc8601-50和California Kid同源性为95.1%~98.4%,与3株Mccp97097ET、Gabes和F38的同源性为99.6%~99.8%。通过对中国分...

Abstract:

Contagious caprine pleuropneumonia (CCPP) is caused by Mycoplasma capricolum subsp.capripneumoniae (Mccp). The aims of this study were to identify 4 Chinese isolated strains employing molecular methods and to determine the appropriate subspecies classification of these strains. Three genome fragments (A，B and C) from each strain were amplified and then transformed into plasmids. The inserted fragments were sequenced and analyzed by comparison with six members of the Mycoplasma mycoides cluster. Cleavage of the PCR products of the 4 strains with PstI yielded three fragments 548，420 and 128bp in length，just like strain F38. The other M. mycoides cluster members had only 2 fragments of 428 and 128bp. Homology analysis of fragment B indicated that the 4 strains exhibited 99.5% homology with Mccp reference strain F38，98.9% with M. capricolum subsp. Capricolum (Mcc) strain California Kid，and only 95.4% with Mmc strain ZZ. In fragment C，the 4 strains had 67.4%～67.6% homology with Mmc PG3，95.1%～98.6% with Mcc strains 8601-50 and California Kid，99.6%～99.8% with Mccp strains 97097ET，Gabes and F38. The analysis revealed that 4 pathogeny strains，87001，87002，367，1653，isolated from China are more closely related to Mccp than to Mcc. Therefore the pathogeny of CCPP in China should be reclassified as Mccp.