蝎子肠道内微生物多样性研究

Microbial diversity in scorpion intestine (Buthus martensii Karsch)

摘要:

蝎子是一种重要的药用动物，还具有很高的营养价值。分别采用非培养和纯培养方法研究蝎子肠道内的微生物群落，结果表明，非培养方法检测到的蝎子肠道内微生物大部分属于α, β, γ-Proteobacteria类群，纯培养法分离到的菌株多属于高G+C含量的革兰氏阳性菌, 两种方法都检测到肠杆菌属（Enterobacter）、沙雷氏菌属（Serratia）、苍白杆菌属(Ochrobactrum)菌株, 综合两种方法检测结果，蝎子肠道微生物共包括23个属，分别是肠杆菌属（Enterobacter）、沙雷氏菌属（Serratia）、假单胞菌属(Pseudomonas)、不动杆菌属(Acinetobacter)、气单胞菌属(Aeromonas)、柠檬酸杆菌属（Citrobacter）、土地杆菌属(Pedobacter)、代尔夫特菌属(Delftia)、罗尔斯通氏菌属(Ralstonia) 、苍白杆菌属(Ochrobactrum)、鞘鞍醇单胞菌属（Sphingomonas）、微小杆菌属（Exiguobacterium ）、戈登氏菌属（Gordonia）、诺卡氏菌属（Nocardia）、红球菌属（Rhodococcus）、两面神菌属（Janibacter）、考克氏菌属(Kocuria)、微球菌属（Micrococcus）、壤霉菌属（Agromyces）、微杆菌属（Microbacterium）、土壤球菌属（Agrococcus）、异常球菌属（Deinococcus）、鸟氨酸微菌属（Ornithinimicrobium），还有一些属于不能培养的未知菌。

Abstract:

Scorpion is an important officinal animal, and has a high nutritional value. In this study, the culture-independent and culture-dependent methods were used to investigate the microbial diversity in the scorpion's intestine. Results based on culture-independent method showed the bacteria to be related to α, β, γ-proteobacteria. Bacteria isolated by the culture-dependent method were high G+C, gram-positive bacteria. The genera Enterobacter, Serratia and Ochrobactrum were detected by both methods. To sum up the results from the two methods, the bacteria in scorpion intestine belong to 23 genera, which are Enterobacter、Serratia、Pseudomonas、Acinetobacter、Aeromonas、Citrobacter、Pedobacter、 Delftia、Ralstonia、Ochrobactrum、Sphingomonas、Exiguobacterium、Gordonia、Nocardia、Rhodococcus、Janibacte、Kocuria、Micrococcus、Agromyces、Microbacterium、Agrococcus、Deinococcus、Ornithinimicrobium, and some uncultured species. The two methods have both advantages and shortcomings. However, when used simultaneously, they complement each other．