链霉菌对烟草中烟碱与绿原酸的降解作用研究

Study on Streptomycetes degradation of nicotine and chlorogenic acid in tobacco

摘要:

为了研究对烟草中烟碱与绿原酸的快速生物降解,筛选了能有效降解烟草中烟碱与绿原酸的链霉菌Z6菌株与Z8菌株,考察了Z6和Z8菌株在不同培养基上的培养特征,探讨了所选菌株对烟碱与绿原酸的降解特性。实验结果表明,Z8链霉菌在烟草固体培养基中培养48h后,对培养基中烟碱的降解率达到83.9%;培养72h后,对烟碱的降解率可达到93.7%,此时烟草中的烟碱含量降低到0.38mg/g,达到了欧盟条例的无害化标准。Z6菌对绿原酸的降解程度较高,培养48h后,对绿原酸的降解率为57.1%;培养72h后,降解率可达到67.5%.

Abstract:

To study the fast bio-degradation of nicotine and chlorogenic acid in tobacco residues, the effective streptomycetes on degradation of nicotine and chlorogenic acid were screened. The cultural characteristics of Z6 and Z8 strains on different media were inspected. The bio-degradation characters of two strains were discussed. The results showed that the nicotine degradation rate of streptomycete Z8 reached to 83.9% after cultured for 48h in tobacco solid media and reached to 93.7% after cultured for 72h, when the nicotine content of tobacco reduce to 0.38mg/g, which is lower than the innocuity standard (European Union Regulations and Italian law classify them as “toxic and hazardous wastes" when the nicotine content exceeds 500 mg/kg d. w.). Z6 is a good strain for chlorgenic acid bio-degradation. The chlorogenic acid degradation rate of Z6 is 57.1% for 48h and 67.5% for 72h respectively after cultured.