茄尼醇转化高产辅酶Q10菌株的分离鉴定与发酵条件的研究

Isolation, Characterization and Fermentation Condition of Coenzyme Q10 Producing Strain with Solanesol as Precursor

摘要:

采用以异戊二烯为唯一碳源的选择性平板筛选模型, 从钱塘江沿岸杭州市九堡段土壤中新筛选到一株产辅酶Q10的细菌菌株E03, 经形态、生理生化、Biolog碳源利用试验和16S rDNA序列分析, 确定 E03属于鞘氨醇属(Sphingomonas sp.), 命名为Sphingomonas sp. ZUTE03。摇瓶试验确定了该菌发酵生产辅酶Q10的最佳碳源为葡萄糖15 g/L, 氮源为硫酸铵10 g/L, 初始pH8.0, 发酵温度25℃, 并考察了该菌转化茄尼醇产辅酶Q10的发酵工艺, 以合适溶剂为溶解体系, 于发酵培养基中摇床培养12 h后, 加入终浓度为0.75 g/L的茄尼醇粗品, 转化12 h, 辅酶Q10产值可达96.88 mg/L。

Abstract:

A bacterium capable of producing Coenzyme Q10 (CoQ10, was isolated from soil. Based on analysis of the 16S rDNA sequence, traditional physiological characteristics, and Biolog-GN, the strain was belonging to the genus Sphingomonas and named as Sphingomonas sp. ZUTEO3. The optimum fermentation condition of CoQ10 production was as following: glucose 15 g/L, (NH4)2SO4 10 g/L, original pH 8.0 and at 25℃. The addition of solanesol could improve CoQ10 production. The optimal condition for the bioconservation from solanesol to CoQ10 was as following: adding 0.75 g/L of raw solanesol after the first 12 h fermentation and continued for another 12 h fermentation. The maximal yield of CoQ10 reached 96.88 mg/L.