酸性和碱性土壤新分离的放线菌中线型和环型质粒的检测

Detection of linear and circular plasmids among actinomycetes isolated from acid and alkaline soils

摘要:

从湖南、云南采集的19份酸性土壤样品分离到50株放线菌, 在pH4.5和pH7.0的培养基上都能生长。从新疆、青海、云南的14份碱性土壤样品中分离到38株放线菌, 在pH10.0和pH7.0的培养基上均能生长。因此, 这些菌株属于中度嗜酸或中度嗜碱放线菌。利用脉冲电泳技术和普通凝胶电泳在其中12株放线菌中检测到线型质粒, 其中3株放线菌中检测到环型质粒。这是首次在中度嗜酸和中度嗜碱的放线菌中报道线型和环型质粒。

Abstract:

Fifty actinomycete strains isolated from 19 acid soil samples collected from Hunan and Yunnan provinces could grow on media at pH4.5 and pH7.0. Thirty-eight actinomycete strains isolated from 14 alkaline soil samples collected from Xinjiang, Qinghai and Yunnan provinces could grow on media at pH10.0 and pH7.0. Thus, they were moderately acidophilic or moderately alkalophilic actinomycetes. Twelve linear plasmids and 3 circular plasmids were detected among the 88 strains by the pulsed-field gel electrophoresis and standard alkaline-denaturation procedure, respectively.