里氏木霉(Trichoderma reesei)分泌组的预测及分析

Prediction and Analysis of the Secreteomic in Trichoderma reesei

摘要:

【目的】里氏木霉是一种重要的产纤维素酶工业用菌种, 研究其分泌组特性具有现实意义。【方法】应用生物信息学方法对里氏木霉基因组中9997个开放阅读框(ORF) 所编码的氨基酸序列进行了分析, 获得了294条可能的分泌蛋白序列, 并且按功能对其进行了分类, 同时用搜索模体的方法在未知功能的序列中找到具有关键模体的序列, 初步确定其潜在的功能。对获得的分泌蛋白的信号肽序列进行了分析。【结果】里氏木霉分泌组中有188种水解酶, 包括114种糖苷水解酶、42种蛋白水解酶和11种脂类水解酶等; 在糖苷水解酶中包括已报道的22种纤维素酶和15种几丁质酶等, 以及30条具有潜在纤维素酶功能的蛋白序列。信号肽序列分析结果表明其同源性较低, 而在信号肽酶切位点附近则相对保守。【结论】通过该预测和分析开拓了里氏木霉的研究空间, 为今后的研究奠定了理论基础。

Abstract:

[Objective] We studied the secretomics’ properties of Trichoderma reesei, an important industrial microorganism used for cellulase production. [Methods] We analyzed the amino acid sequences coded by 9997 ORFs in Trichoderma reesei genome with bioinformatics approaches, identified 294 possible secreted protein sequences, and classified them by functions. We also applied motif search methods to search key motifs in the function-unknown sequences and preliminary predicted their functions. Moreover, we analyzed the signal peptide sequences of the secreted proteins. [Results] There were 188 hydrolytic enzymes in Trichoderma reesei’s secretomics, including 114 glycosidases, 42 proteases, and 11 li-poidases. The glycosidases included 22 reported cellulases, and 15 chitinases, as well as 30 other protein sequences probably related to cellulose degradation. The homology of signal peptides of secreted proteins was low whereas se-quences near the digesting site of signalase were conservative. [Conclusion] This method gave insights into the whole secreted proteome of Trichoderma reesei and provided basis for further studies on secretomic features at a genome level.