一株寄生油松毛虫的白僵菌毒素化学成分

Isolation and identification of toxins inhibiting Dentrolimus tabulaeformis from an antagonistic strain of Beauveria

摘要:

【目的】从一株寄生于油松毛虫（Dentrolimus tabulaeformis Tsai et Liu）越冬幼虫上的高毒力卵孢白僵菌(Beauveria tenella )的发酵液中，分离纯化出对油松毛虫幼虫具有杀虫活性的毒素物质。【方法】对这株白僵菌进行了液体培养，用乙酸乙酯对发酵液进行萃取，然后对粗提物进行了硅胶色谱分离, 利用GC/MS对第6组物质进行化学成分分析。【结果】其发酵液的乙酸乙酯粗提物对油松毛虫具有杀虫活性（校正死亡率42.52%）对粗提物进行了硅胶色谱分离，共得到6组物质，其中第6组物质对油松毛虫的校正致死率达80.26%。利用GC/MS对第6组物质进行化学成分分析，得知其包括17个组分，其中相对含量大于10%的有：2-哌啶酮占14.02%，2-香豆满酮占47.10%，六氢化-吡咯环[1,2-a] 吡嗪 -1, 4- 二酮占21.05%。用其中的2-哌啶酮和2-香豆满酮分别作杀虫试验，油松毛虫的校正死亡率分别为83.32% 和91.61%。【结论】2-哌啶酮和2-香豆满酮是该白僵菌菌株的代谢毒素物质。

关键词:油松毛虫;卵孢白僵菌;毒素;2-哌啶酮;2-香豆满酮

Abstract:

[Objective] We used submerged fermentation to cultivate a strain of the entomopathogenic fungus Beauveria tenella isolated from the infected larvae of Dentrolimus tabulaeformis in Pinus tabulaeformis forest in Chengde of Hebei Province in China [Methods] We used ethyl acetate to extract antagonistic components from the fermentation broth and used silica gel column chromatography and GC/MS to separate and identify the components. [Results] Six compounds were obtained by silica gel column chromatography. The sixth compound had higher activity to kill the larvae of Den-trolimus tabulaeformis with a corrected mortality rate of 80%. Seventeen compounds were separated and identified by GC/MS in the 6th group, of which 3compounds were more than 10%, 2-Piperidinone(14.02%), 2-coumaranone(47.10%), and Pyrrolo[1,2-a]Pyrazine-1,4-dione, hexahydro (21.05%). [Conclusion] 2-Piperidinone and 2-coumaranone had insec-ticidal activity (corrected mortality rate reached 83.32% and 91.61% respectively) and were the most important toxic sub-stances to control pests.

Key words:Dentrolimus tabulaeformis; Beauveria tenella; toxin; 2-Piperidinone; 2-coumaranone