一种改进的平板影印工具及其应用

An improved replica plating method and its application to the high-throughput selection of transformants

摘要:

【目的】建立一种简便高效的平板影印工具，克服传统影印工具的缺陷。【方法】将大量竹签按照一定方法捆扎成与平皿内盖直径相近的捆，将单根牙签转移菌落的效果进行扩大，从而实现菌落的大规模转移。【结果】本实验室使用上述工具成功地进行了平板影印和传代，并进行转化子大规模筛选。【结论】与传统工具相比，该影印工具更为经济简便，而且效果清晰，为微生物平板筛选提供新的方法和思路。国家发明专利申请号：2007100291331。

关键词:微生物;平板影印;高效筛选

Abstract:

[Objective] To make a simpler and highly efficient replica plating tool to avoid the limitations of the traditional method. [Methods] A number of bamboo-toothpicks was sheaved to form a bundle with diameter close to the plate diameter. The sheaved bamboo-toothpicks were used for replica-plating. [Results] This replica plating tool was successfully used for the screening of Saccahromyces cerevisiae transformants. [Conclusion] Compared with the traditional tool, this tool was simpler and highly efficient.

Key words:microbiology; replica plating; highly efficient selection