植物乳杆菌ZJ316生产细菌素

College of Food & Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China

摘要:

【目的】研究植物乳杆菌ZJ316生长和产细菌素的最佳培养基成分和发酵条件，以提高该菌产plantaricin ZJ316的能力。【方法】 改变培养基成份和发酵条件，考察不同氮源、碳源等培养基成分和不同的发酵温度等条件对ZJ316生长和产细菌素的影响。【结果】 最佳培养基为MRS培养基；优化后的培养基配方为葡萄糖10 g/L，麦芽糖10 g/L，酵母提取物10 g/L，蛋白胨10 g/L，柠檬酸三铵2 g/L，吐温80为1 mL/L，K2HPO4·3H2O 6 g/L，乙酸钠5 g/L，硫酸镁0.2 g/L，硫酸锰0.05 g/L。培养基初始pH6.5，30℃静置培养24 h。【结论】通过培养基成分和发酵条件的优化，细菌素产量提高了2.3倍，为进一步研究和规模化生产奠定基础。

Abstract:

[Objective] To enhance the production of plantaricin by Lactobacillus plantarum ZJ316 isolated from infant feces. [Methods] We analyzed fermentation parameters influencing cell growth and plantaricin production with different medium composition and under different cultivation conditions. [Results] MRS (DE MAN, ROGOSA, SHARPE) medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4 ? 3H2O, 5 g/L sodium acetate, 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. The optimal initial pH and temperature for plantaricin production were 6.5 and 30℃ for 24 h. [Conclusion] After optimizing, the production of plantaricin was increased 2.3-fold using the optimized medium, compared with in the basic MRS medium.