乙肝病毒感染导致Mrell下调及基因组断裂

HBV infection downregulated Mre11 expression and induced genome instability

摘要:

【目的】乙肝病毒的持续感染与肝细胞癌的发生密切相关。本文探讨了乙肝病毒感染后导致宿主蛋白Mre11的变化，并研究这种蛋白的变化可能导致肝癌发生的机制。【方法】本文通过Western blot检测了乙肝病毒感染HL7702细胞及肝癌组织标本的Mre11蛋白的变化，并且通过RNA干涉的方法干扰了Mre11的表达，用连接介导PCR检测基因组的变化。【结果】本研究发现乙肝病毒感染细胞导致Mre11表达下调，肝癌组织也可以发现Mre11表达下调；下调Mre11表达可以导致细胞基因组的断裂增多。【结论】HBV感染导致Mre11表达下调导致基因组不稳定，这可能与HBV感染导致细胞恶性转化相关。

Abstract:

[Objective] Prolonged infection with Hepatitis B virus (HBV) has been recognized as a major factor for hepatocellular carcinoma (HCC). In this study, we studied the host protein Mre11 fluctuations after HBV infection which might be eventually contributed to cell transformation. [Methods] Western Blot was monitored to detect the expression of Mre11, and RNA interference was used to downregulate protein expression. Then, Ligation mediated PCR was used to detect the level of DNA double strand breaks. [Results] Mre11 protein was downregulated when HBV infection occured, and the downregulated expression was also seen in HCC tissue. By RNA interference, we found that Mre11 knockdown caused DNA instability. [Conclusion] Mre11 expression downregulation contributed at least partially to cell transformation caused by HBV infection.