铜绿假单胞菌中一个新的吩嗪合成调节基因

A novel transcriptional regulator of the phzA1 operon inPseudomonas aerugi-nosa

摘要:

【目的】在次抑制浓度四环素条件下，研究铜绿假单胞菌phzA1操纵子的调节基因及调节途径。【方法】对转座突变库中phzA1操纵子表达发生变化的突变体，进行随机PCR、基因测序及比对，确定突变位点。并以发光杆菌的荧光素酶基因操纵子luxCDABE为报道基因，研究基因调节作用及调节路径。【结果】在两株突变体PAM0487和PAM0487R中phzA1操纵子的表达降低，这两株突变体的突变基因确定为假定钼元素转运蛋白调节子PA0487基因。【结论】PA0487是phzA1操纵子表达的一个新的正向调节子，并对密度感应系统相关基因的表达有调节作用。

Abstract:

[Objective] The aim was to investigate the regulator of phzA1B1C1D1E1F1(phzA1) operon in Pseudomoas aeruginosa. [Methods] Mutants affecting phzA1 expression in the presence of subinhibitory concentrations of tetracyclin were selected by screening a transposon mutagenesis library. The transposon insertion sites in the mutants were determined by arbitrary PCR and subsequent PCR product sequencing. [Results] We found two mutants affecting the phzA1 operon expression. The transposon insertion sites in these two mutants were at the promoter region of gene PA0487 encoding a probable molybdenum transport regulator. Some genes involved in quorum sensing were also down-regulated in the mutant. [Conclusion] PA0487 was involved in regulating the expression of phzA1, possibly through the quorum sensing system.