链霉菌Z314的分类鉴定及其转化产物普伐他汀的纯化研究

Classification of Streptomyces strain Z314 and purification of its product pravastatin

摘要:

从土壤中筛选得到1株可转化美伐他汀(compactin)生产普伐他汀(pravastatin)的放线菌Z314, 根据形态观察、培养特征、生理生化鉴定以及16S rRNA序列分析, 初步判定该菌株为链霉菌属中的黄质产色链霉菌(Streptomyces xanthochromogenes)。经发酵条件优化, 该菌株转化美伐他汀生产普伐他汀的产量可达1580mg/L, 转化率为49.45%。发酵液经中空纤维素过滤、大孔树脂吸附和HPLC纯化, 普伐他汀的回收率为45.06%, 纯度为99.02%, 为进一步产业化开发奠定了基础。

Abstract:

Actinomycete strain Z314 with the capability of converting compactin to pravastatin was isolated from soil samples. Based on the results of morphological, physiological, chemotaxonomic characteristics and 16S rRNA analysis, strain Z314 was placed within the genus Streptomyces and identified as the species Streptomyces xan-thochromogenes. The production of pravastatin reached 1580mg/L under optimized feeding of compactin, and the conversion rate was 49.45%. With the efficient purification system by fibrin column, macroporous adsorption resin and high performance liquid chromatography, pravastatin was purified from the culture supernatant with an overall recovery of 45.06% and purity of 99.02%. It is the first report in the world to produce pravastatin by con-verting compactin using Streptomyces xanthochromogenes under optimized conditions, the conversion of compactin to pravastatin was higher than other methods reported. The recovery and purity were improved by the three-step purification of pravastatin from the culture supernatant.