白念珠菌高铁还原酶FRP1基因的功能

Ferric reductase FRP1 gene in Candida albicans

摘要:

摘要: 白念珠菌（Candida albicans）获得铁的能力影响细胞的生长和毒力，高铁还原酶是白念珠菌高亲和铁吸收系统的重要组成部分。【目的】构建高铁还原酶FRP1（Ferric reductase protein）基因缺失突变株，对FRP1基因功能进行初步研究。【方法】使用Northern杂交的方法分析FRP1基因在缺铁和富铁条件下的表达。利用PCR介导的基因敲除技术构建frp1缺失突变株，并且对野生型和缺失突变株在细胞高铁还原酶活性以及缺铁条件下的生长情况进行比较分析。【结果】缺铁条件可以诱导FRP1基因的表达。frp1缺失突变株不能在铁缺陷的固体培养基上生长。【结论】FRP1蛋白可能是白念珠菌在缺铁条件下起主要作用的高铁还原酶。

Abstract:

Abstract: The ability of iron acquisition in Candida albicans has an effect on its growth and pathogenesis. Ferric reductases are important components of high affinity iron acquisition system in C. albicans. [Objective] The aim of this study was to elucidate the function of FRP1 (Ferric reductase protein). [Methods] FRP1 gene expression was detected under low-iron and high-iron conditions by Northern blot. We used the method of PCR-directed gene disruption to construct frp1 null mutant and then the phenotypes of frp1Δ/Δ mutant were characterized. [Results] Low-iron condition induced FRP1 gene expression. frp1Δ/Δ mutant showed no growth on low-iron media compared to wild-type strains on solid plates. [Conclusion] FRP1 protein is probably the main ferric reductase under low-iron condition.