J亚群禽白血病病毒蛋鸡分离株SD07LK1全基因组核苷酸序列的比较

Sequence analysis for the complete proviral genome of Avian Leukosis Virus（Subgroup J）strain SD07LK1 isolated from layers

摘要:

摘要： 【目的】分析我国ALV-J蛋鸡分离株的来源和进一步演变趋势。【方法】以J亚群禽白血病病毒(ALV-J)蛋鸡分离株SD07LK1感染的鸡胚成纤维细胞(CEF)基因组DNA作为其前病毒基因组模板，根据已发表序列设计合成9对引物, 经PCR扩增出9段连续的、相互部分重叠的DNA片段和闭合环形前病毒两末端LTR的连接区段，并分别连入T载体进行克隆、测序。【结果】用DNAstar软件对测序结果进行剪辑和拼接，首次完成了ALV-J蛋鸡分离株SD07LK1的前病毒全基因组核苷酸序列。【结论】将该序列与另外已完成的全基因组序列的比较表明，ALV-J的整个基因组gag和pol基因相对保守，各毒株间对应基因的同源性分别在95.0%以上，env基因的同源性仅为88.6-94.0%。

Abstract:

Abstract: [Objective]To study the source and trend in evolution of ALV-J strains from layers in China. [Methods]The genomic DNA extracted from chicken embryo fibroblasts (CEF) infected by ALV-J strain SD07LK1 isolated from layers was used as template to amplify the ALV-J proviral DNA by PCR. Nine continuous and overlapping fragments were amplified with nine pairs of primers according to published sequences, then cloned into the T vector and sequenced. [Results]The complete sequence of the whole genome of ALV-J Chinese strain SD07LK1 was first established. [Conclusion]Comparisons of SD07LK1 sequence with that of the other Avian leukosis viruse strains, by using DNAstar software, demonstrated that the genes gag and pol of ALV-J were relatively conservative, the nucleotide identity of all the strains was over 95.0%. However, the gene env identity was only in the ranges between 88.6 and 94.0%.