中国家猫克氏巴尔通体分离株生物学及分子特征分析

Biological and molecular characteristics of a cat-borne Bartonella clarridgeiae

摘要:

摘要：【目的】分析中国家猫中分离的巴尔通体菌株M9HN-SHQ生物学性状和分子特征。【方法】应用含5％羊血的胰酶大豆琼脂培养基在5％ CO2培养箱中37℃培养6～7 d，革兰氏和吉姆尼茨染色镜下观察形态；应用VITEK ANI厌氧菌鉴定卡进行生化反应鉴定；气相色谱分析方法获取菌体脂肪酸成份组成（CFA）；Etest药敏试条测定对10种抗生素的敏感性；分别应用随机扩增多态性DNA（RAPD）和脉冲场凝胶电泳（PFGE）技术对目标菌株和其他国际标准菌株等绘制DNA指纹图谱；对16S rRNA，gltA，gro

Abstract:

Abstract : [Objective] To characterize a Bartonella strain M9HN-SHQ from a blood culture of cat from Henan Province, China. [Methods] The organisms were subcultured in 5% CO2 at 37℃ on trypticase soy agar containing 5% sheep blood for 6 to 7 days. We analyzed the isolate using whole-cell fatty acid analysis, Etest for susceptibility testing, random amplified polymorphic DNA(RAPD), pulsed-field gel electrophoresis(PFGE) and sequence analysis of 16S rRNA, gltA, groEL, ftsZ, rpoB, ribC and 16S～23S rRNA intergenic spacer region. [Results] Isolate M9HN-SHQ stained faintly as a gram-negative rod but was easier to visualize when stained by the Giménez technique. Most of the biochemical and cellular fatty acid properties of strain M9HN-SHQ were typical for bacteria of the Bartonella genus. The strain was susceptible to Cefotaxime sodium, Rifampin, Ciprofloxacin and other four antibiotics. Genotypic characterization of strain M9HN-SHQ, including RAPD, PFGE was distinguishable from the reference strains of B. henselae, B. elizabethae, B. vinsonii subsp. berkhoffii and B. grahamii. Sequence analysis of the genes from the seven chromosomal regions identified the strain M9HN-SHQ as B. clarridgeiae. [Conclusion] To our knowledge, this is the first report that documents Bartonella clarridgeiae infections of domestic cats in China.