表达SLTEC保护性抗原的重组猪霍乱沙门氏菌C500株的构建及生物学特性

Construction of attenuated Salmonella choleraesuis vaccine strain expressing recombinant antigen of Shiga-like toxin Escherichia coli

摘要:

摘要:【目的】 利用平衡致死系统构建表达产类志贺氏毒素大肠杆菌(Shiga-like toxin Escherichia coli , SLTEC)保护性抗原的减毒猪霍乱沙门氏菌。【方法】 构建表达SLT-IIeB－FedF的重组质粒 ,再将其电转入终宿主菌减毒猪霍乱沙门氏菌ΔasdC500株中构建成口服活疫苗株 ,经聚丙烯酰胺凝胶电泳检测SLT-IIeB－FedF融合蛋白的表达情况,并观察重组菌体外培养的稳定性。【结果】 利用宿主-载体平衡致死系统构建了表达SLTEC保护性抗原的重组减毒猪霍乱沙门氏菌

Abstract:

Abstract: [Objective] To construct an attenuated Salmonella choleraesuis vaccine strain expressing the gene SLT-IIeB and FedF of Shiga-like toxin Escherichia coli (SLTEC) O138 with balanced lethal system. [Methods] The gene of SLT-IIeB and FedF of SLTEC O138 was amplified , and then recombined with a vector pYA3493 (Asd+). The recombinant was electroporated into attenuated Salmonella choleraesuis C500(Asd-). The expression of SLT-IIeB and FedF was analyzed by SDS-PAGE. The stability of the vaccine strains was studied by generation culture in vitro. [Results] The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB. [Conclusion] The attenuated Salmonella choleraesuis vaccine strain could probably serve as a vaccine against edema disease and piglets paratyphoid.