Hongyue Zhai
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaLihong Tao
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaJinying Ge
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaXijun Wang
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaNa Feng
Institute of Military Veterinary，Academy of Military Medical Sciences，Changchun 130122，ChinaLei Shuai
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaLiang Ma
School of Veterinary Medicine，Northeast Agricultural University，Harbin 150030，ChinaZhigao Bu
Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology， Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences，Harbin 150001，ChinaSupported by the National Science Major Technology Projects(2009ZX10004-214)
Abstract: ［Objective］To study the biological characteristics and pathogenicity of a recombinant rabies virus Flury LEP (low egg passage) that has two glycoprotein genes (Ggene)．［Methods］By using reverse genetics techniques，we constructed a recombinant virus Flury LEP that has an additional G gene between P and M gene (rLEP-PGM)．Then we studied the biological characteristics of the recombinant virus and its pathogenicity on mice．［Results］The in vitro growth characteristic of rLEP-PGM were similar to the LEP strain． Western blot analysis of glycoprotein expression showed that the glycoprotein expression level of rLEP-PGM was 1.5 times higher than LEP． The LD50 of rLEP-PGM and LEP was 3 FFU and 1 FFU by intracerebral injection． However，the LD50 of intramuscular injection was 4×104 Lg FFU and 3.2×105 Lg FFU，respectively．［Conclusion］Insertion of an additional G gene between P and M gene can significantly raise the expression level of glycoprotein and enhance the ability to invade central nervous system from peripheral sites．
Hongyue Zhai, Lihong Tao, Jinying Ge, Xijun Wang, Na Feng, Lei Shuai, Liang Ma, Zhigao Bu. Insertion of glycoprotein gene between P and M gene influences the pathogenicity of the rabies virus Flury LEP. [J]. Acta Microbiologica Sinica, 2011, 51(8): 1098-1105
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