High-throughput screening of human pancreatic α-amylase inhibitors

Abstract:

Abstract: ［Objective］ Targeting the important enzyme in human glucose metabolic pathway，we established a high throughput screening model for human pancreatic α-amylase inhibitors． ［Methods］ Pichia pastoris expression system was used to clone and express the human pancreatic α-amylase; we established the α-amylase inhibitor screening model using the catalytic properties of enzyme; this model was applied in screening of actinomycete' metabolites; the taxonomic status of positive strains were analyzed by constructing 16 S rRNA phylogenetic tree． ［Results］ We cloned and expressed the intact gene of human pancreatic α-amylase successfully; the high-throughput screening model of α-amylase inhibitors was established; nearly 2000 actinomycete' metabolites were screened，14 α-amylase inhibitor producing strains were obtained finally， and showed taxonomically rich diversity． ［Conclusion］ The α-amylase inhibitor high-throughput screening model had high practical value for developing new hypoglycemic drugs．

Key words:Keywords: α-amylase inhibitor，Pichia pastoris expression system，human pancreatic α-amylase，actinomycetes，highthroughput screening