Yanzhou Gao
Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaHongxia Fan
Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaLipeng Qiu
University of Chinese Academy of Sciences，Beijing 100049，ChinaSongdong Meng
Chinese Academy of Sciences Key Laboratory of Pathogenic Microbiology and Immunology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaSupported by the National Science and Technology Major Project (2013ZX10002001-003-003) and by the Shenzhen Science and Technology Innovation Commission Project(KQA201105260010A，CYC201012290024A)
Abstract:［Objective］To investigate the effect of the Interferon-α-induced gp96 upregulation on the anti-HBV efficiency of Interferon-α(IFN-α).［Methods］The effect of IFN-α on the transcription and expression of heat shock protein gp96 was determined by real-time PCR，luciferase reporter assay and Western blot．The effect of over-expression or knock-down of gp96 by transfection or RNA interference，and treatment with IFN-α on HBV expression and replication was examined by ELISA and RT-PCR.［Results］IFN-α treatment led to increased gp96 expression in a time- and dose-dependent manner. The overexpression of gp96 enhanced HBV expression and replication，whereas downregulation of gp96 resulted in decreased HBV replication． Finally，we verified that blocking IFN-α-induced upregulation of gp96 significantly enhanced IFN-α-mediated HBV inhibitory effects.［Conclusion］ IFN-α-induced upregulation of gp96 may negatively affect the anti-HBV function of IFN-α. These data provide valuable insight for enhancing the antiviral efficacy of IFN-α by simultaneous inhibition of gp96．
Yanzhou Gao, Hongxia Fan, Lipeng Qiu, Songdong Meng. IFN-α-induced gp96 upregulation negatively affects the anti-HBV efficiency of IFN-α. [J]. Acta Microbiologica Sinica, 2013, 53(8): 867-874
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