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微生物学通报

我的厌氧菌研究之路与心得
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国家自然科学基金重点项目(31430001);国家自然科学基金重大研究计划(92251302)


My research road and experience on anaerobes
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    摘要:

    厌氧微生物,尤其是产甲烷古菌,广泛分布于各种缺氧环境中,它们具有丰富多样的代谢途径,在地球碳氮等重要物质循环及维持环境健康中发挥着不可或缺的作用。厌氧菌不仅是人肠道微生物组的主要成分,而且是将废物降解转化为生物能源——甲烷的驱动者。我从认识厌氧微生物,尤其是产甲烷古菌,到毕生对它们的研究,既有心得又有经验教训,愿在《微生物学通报》建刊50周年之际,分享给致力于微生物研究的青年才俊们,希望对你们有所借鉴。

    Abstract:

    Anaerobic microorganisms, particularly methanogens, are widely distributed in the anoxic environments of earth. They have diverse metabolic potentials, and play indispensable roles in earth materials cycle and maintaining environmental and human healthy. Anaerobes are not only the main species in human intestinal microbiome, but also are the players converting organic waster to bioenergy, methane. I have been engaged in anaerobes especially in methanogenic archaea for more than 30 years, and would like to share my experience and lessons in the research on anaerobes with the young microbiologists at the time of the 50th anniversary of Microbiology China.

    参考文献
    [1] SP Lapage, PHA Sneath, EF Lessel, VBD Skerman, HPR Seeliger, WA Clark. International Code of Nomenclature of Bacteria[M]. Washington (DC): ASM Press, 1992.
    [2] JIAN W, ZHU L, DONG X. New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences[J]. International Journal of Systematic and Evolutionary Microbiology, 2001, 51(Pt 5): 1633-1638.
    [3] LI J, ZHENG XW, LI LY, ZHANG SJ, REN MF, HUANG L, DONG XZ. The archaeal transcription termination factor aCPSF1 is a robust phylogenetic marker for archaeal taxonomy[J]. Microbiology Spectrum, 2021, 9(3): e0153921.
    [4] ZHANG GS, ZHANG F, DING G, LI J, GUO XP, ZHU JX, ZHOU LG, CAI SC, LIU XL, LUO YM, ZHANG GF, SHI WY, DONG XZ. Acyl homoserine lactone-based quorum sensing in a methanogenic archaeon[J]. The ISME Journal, 2012, 6(7): 1336-1344.
    [5] MA K, LIU XL, DONG XZ. Methanosaeta harundinacea, sp. nov., a novel acetate-scavenging methanogen isolated from a UASB reactor[J]. International Journal Systematic and Evolutionary Microbiology, 2006, 56: 127-131.
    [6] LI J, ZHENG X, GUO XP, QI L, DONG XZ. Characterization of an archaeal two-component system that regulates methanogenesis in Methanosaeta harundinacea[J]. PLoS One, 2014, 9(4): e95502.
    [7] LI LY, ZHENG MY, MA HL, GONG SF, AI GM, LIU XL, LI J, WANG KJ, DONG XZ. Significant performance enhancement of a UASB reactor by using acyl homoserine lactones to facilitate the long filaments of Methanosaeta harundinacea 6Ac[J]. Applied Microbiology and Biotechnology, 2015, 99(15): 6471-6480.
    [8] CHEN C, LI LY, HUANG K, ZHANG J, XIE WY, LU YH, DONG XZ, ZHAO FJ. Sulfate-reducing bacteria and methanogens are involved in arsenic methylation and demethylation in paddy soils[J]. The ISME Journal, 2019, 13(10): 2523-2535.
    [9] CHEN C, LI LY, WANG YF, DONG XZ, ZHAO FJ. Methylotrophic methanogens and bacteria synergistically demethylate dimethylarsenate in paddy soil and alleviate rice straighthead disease[J]. The ISME Journal, 2023, 17(11): 1851-1861.
    [10] QI L, YUE L, FENG DQ, QI FX, LI J, DONG XZ. Genome-wide mRNA processing in methanogenic Archaea reveals post-transcriptional regulation of ribosomal protein synthesis[J]. Nucleic Acids Research, 2017, 45(12): 7285-7298.
    [11] JIA J, LI J, QI L, LI LY, YUE L, DONG XZ. Post-transcriptional regulation is involved in the cold-active methanol-based methanogenic pathway of a psychrophilic methanogen[J]. Environmental Microbiology, 2021, 23(7): 3773-3788.
    [12] ZHANG B, YUE L, ZHOU LG, QI L, LI J, DONG XZ. Conserved TRAM domain functions as an archaeal cold shock protein via RNA chaperone activity[J]. Frontiers in Microbiology, 2017, 8: 1597.
    [13] LI J, ZHANG B, ZHOU LG, QI L, YUE L, ZHANG WT, CHENG HC, WHITMAN WB, DONG XZ. The archaeal RNA chaperone TRAM0076 shapes the transcriptome and optimizes the growth of Methanococcus maripaludis[J]. PLoS Genetics, 2019, 15(8): e1008328.
    [14] 陈巍, 李华丽, 邱金龙. 异源表达古菌TRAM基因增强水稻的耐旱性[J]. 生物工程学报, 2019, 35(9): 1676-1685. CHEN W, LI HL, QIU JL. Ectopic expression of archaeal TRAM-encoding genes in rice improves its drought-tolerance[J]. Chinese Journal of Biotechnology, 2019, 35(9): 1676-1685(in Chinese).
    [15] YUE L, LI J, ZHANG B, QI L, LI ZH, ZHAO FQ, LI LY, ZHENG XW, DONG XZ. The conserved ribonuclease aCPSF1 triggers genome-wide transcription termination of Archaea via a 3'-end cleavage mode[J]. Nucleic Acids Research, 2020, 48(17): 9589-9605.
    [16] ZHANG WT, REN DR, LI ZH, YUE L, WHITMAN WB, DONG XZ, LI J. Internal transcription termination widely regulates differential expression of operon-organized genes including ribosomal protein and RNA polymerase genes in an archaeon[J]. Nucleic Acids Research, 2023, 51(15): 7851-7867.
    [17] LI J, YUE L, LI ZH, ZHANG WT, ZHANG B, ZHAO FQ, DONG XZ. aCPSF1 cooperates with Terminator U-tract to dictate archaeal transcription termination efficacy[J]. eLife, 2021, 10: e70464.
    [18] XU Q, DU Q, GAO J, CHEN L, DONG XZ, LI J. A robust genetic toolbox for fine-tuning gene expression in the CO2-Fixing methanogenic archaeon Methanococcus maripaludis[J]. Metabolic Engineering, 2023, 79: 130-145.
    [19] DU Q, BAI LP, DONG XZ, LI J. An improved CRISPR and CRISPR interference (CRISPRi) toolkit for engineering the model methanogenic archaeon Methanococcus maripaludis[J]. Microbial Cell Factories, 2024. DOI: doi.org/10.1186/s12934-024- 02492-0.
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东秀珠. 我的厌氧菌研究之路与心得[J]. 微生物学通报, 2024, 51(12): 4827-4833

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  • 收稿日期:2024-07-04
  • 录用日期:2024-07-20
  • 在线发布日期: 2024-12-24
  • 出版日期: 2024-12-20
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