L-丝氨酸生产菌的选育及不同碳源对发酵的影响

Study on the Breeding of L-serine-producing Mutant and the Effect of Different Carbon Source

摘要:

以一株黄假单胞属(Pseudomonas flava)菌株A3为出发菌株,经过紫外(UV)诱变和硫酸二乙酯(DES)逐级诱变处理和选育,选育出一株能够以甲醇为唯一碳源的兼性甲基营养型菌JW-01(Mth^R、Gly^R)。在含甘氨酸30g/L、甲醇1%的发酵培养基中发酵3d后L-丝氨酸产量为6．2g/L,较出发菌株提高了67．6%。该菌具有较好的传代稳定性。

关键词:L-丝氨酸; 黄假单胞属; 发酵; 诱变育种

Abstract:

A L-serine producing facuhative methylotroph mutant (Mth^H、Gly^R)was derived from Pseudomonas flava A3 by combination treatment with ultraviolet(UV)and diethylsulfate(DES).It could accumulate 6.2g/L L-serine in the medium containing 30g/L glycine and 1% methanol as carbon source when it was cultured for 3 days,and it has an improvement about 67.6% compared with the origin.The mutant also has a good stability of descendiblity of L-serine producing.

Key words:L-serine; Pseudomonas flava; Fermentation; Breeding