珍稀药用真菌——樟芝深层发酵培养条件的优化

Optimum of Submerged Fermentation Parameters for Antrodia camphorata

摘要:

对樟芝深层发酵培养基进行了筛选,并在此基础上对发酵条件进行了优化。以樟芝深层发酵菌丝体三萜产量为主要目标产物,确定发酵培养条件为:40g/L葡萄糖,6g/L豆饼粉,1g/L K₂HPO₄,0．5g/L MgSO4,VB₁ 100mg/L,自然pH,接种量为20%,装液量为100mL/250mL三角瓶,转速100r/min,26℃恒温培养6d,胞内三萜产量达15．25mg/100mL发酵液。

关键词:樟芝; 三萜; 深层发酵

Abstract:

Optimal parameters of submerged fermentation for Antrodia camphorata were studied.According to the yield of Intracellular triter- pene,the optimal fermentation parameters were obtained as follows:40g/Lglucose,6 g/L soybean,1 g/L K₂HPO₄,0.5 g/L MgSO₄ and 100mg/L VB₁.The optimum cultural period was 6 d at 26℃in 250mL shake flasks at 100r/min.The best aeration ratio was 1:2.5(medi- um volume:flask volume).The inoculation volume was 20%.The intracellular triterpene of Antrodia camphorata was obtained(about 15.25mg/100mL) in the optimum culture.

Key words:Antrodia camphorata; Triterpene; Submerged fermentation