处理甲苯废气的生物滴滤池中微生物生态学研究

Study of Microbial Ecology in Biotrickling Filter Used to Treat Waste Air Containing Toluene

摘要:

利用PCR-DGGE技术分析了处理甲苯废气的生物滴滤池生物多样性，结果表明：在运行过程中，随着生物滴滤池对甲苯去除能力的不断增加,填料当中的微生物种群也发生了明显的变化。在甲苯的选择压力下，随时间的迁延，微生物种类减少，优势种群的相对丰度增加，处于不同层面填料上的微生物分布也趋向于一致。

关键词:生物滴滤池，生物多样性，变性梯度凝胶电泳

Abstract:

Denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16s rDNA was used to assess changes of biodiversity in a biotrickling filter used to treat waste air containing toluene. The results shown that along with gradually increase of removing capacity for toluene, microbial community structure in packing materials sampled from biotrickling filter also changed markedly over periods of experiment. Under selective pressure of toluene, the number of microbial species decreased but relative abundance of some predominant species increased，and microbial spatial location along the height of biotrickling filter tend to be identical.

Key words:Biotrickling Filter, Biodiversity, DGGE