七种我国昆虫病原线虫共生菌的分离与鉴定

Isolation and Identification of Seven Symbiotic Bacteria from Local Entomopathogenic Nematodes

摘要:

昆虫病原线虫共生菌是一类具有广阔发展前景的生物防治资源, 由于这类菌的分类鉴定工作起步晚, 存在分类混乱和不系统的现象, 在本土资源十分丰富的我国尤为突出。本文对本实验室分离的7个共生菌菌株进行了分类描述, 建立了以生理形态, 生化特征为分类基础, 结合16S rDNA序列分析的有效鉴定方法。

关键词:昆虫病原线虫共生细菌, 致病杆菌属, 光杆状菌属, 16S rDNA, 分类鉴定

Abstract:

The symbiotic bacterium exists in the intestines of entomopathogenic nematodes and is a potential biological agent. Systematic classification of these bacteria is scarce in China. In this paper, seven strains of symbiotic bacteria from local entomopathogenic nematodes were identified by both observation of morphology, physiological, biochemical characteristics and sequence analysis of 16S rDNA fragments.

Key words:Symbiotic bacterium of entomopathogenic nematode, Xenorhabdus, Photorhabdus, 16S rDNA, Identification