基因强化在难降解污染物生物处理和修复中的应用

Application of Gene Bioaugmentation in Treatment of Recalcitrant Chemicals

摘要:

基因强化通过强化降解基因在土著菌群中的水平迁移和传播, 促进土著降解菌群的进化, 改善基因工程菌生物强化作用的稳定性, 提高难降解污染物的生物去除效果。介绍了基因强化的原理-微生物群落内水平基因迁移, 讨论了基因载体、细胞接触条件和环境条件等影响基因强化的因素, 综述了目前基因强化在土壤生物修复和废水生物处理中的应用现状, 并提出了基因强化中存在的问题。

关键词:基因强化, 基因工程菌, 生物强化处理, 难降解污染物

Abstract:

One of the new approaches for bioaugmentation treatment of recalcitrant chemicals is gene bioaugmentation, which is helpful to improve the bioaugmentation stability when genetically engineered microorganisms are used. The mechanism of gene bioaugmentation, viz. horizontal gene transfer within microbial community, is introduced. The influence factors of gene bioaugmentation: gene vector, cell touch conditions and environment conditions, are discussed. The applications in soil bioremediation and wastewater treatment of gene bioaugmentation are also summarized. The limitation of gene bioaugmentation is also mentioned.

Key words:Gene bioaugmentation, Genetically engineered microorganisms, Bioaugmentation treatment, Recalcitrant chemicals