树脂吸附法分离瓜果腐霉毒素中的除草活性物质

Colophony Adsorbing Isolating Herbicidal Substance from Mycotoxin Produced by Pythium aphanidermatum

摘要:

本试验以对禾本科杂草具有除草活性的瓜果腐霉菌株PAM1为试验材料, 研究了X-5、AB-8、H-103和S-8等4种大孔吸附树脂对其除草活性物质的吸附性能, 结果表明X-5树脂能够较好地吸附该除草活性物质。选用50%乙醇、95%乙醇、乙酸乙酯和丙酮4种溶剂为供试洗脱溶剂, 采用2种洗脱方案, 对其最佳洗脱溶剂进行了选择, 生长抑制作用和种子萌发试验结果表明, 以方案1洗脱为最佳。

关键词:瓜果腐霉病菌, 除草活性, 树脂柱层析

Abstract:

Four kinds of colophony were used to isolate the herbicidal substance from the toxin produced by isolate PAM1 of Pythium aphanidermatum, which had herbicidal activity to Digtaria sanguinealis L and the results showed X-5 was the best one for absorbing herbicidal substance among the four kinds of colophony used. Four solvents including 50% ethanol, 95% ethanol, ethyl acetate and acetone were used as eluting solvent and 2 procedures were tested, and the results of growth inhibition and seed germination indicated that the best eluting procedure was procedure 1.

Key words:Pythium aphanidermatum, Herbicidal activity, Colophony