食品卫生微生物学检验培养基质量分析

Quality Analysis of Microbial Culture Media for Food Hygienic Detection

摘要:

为探讨培养基ISO国际标准对国内培养基质控的等效性, 选择国内外4家培养基生产厂家的霉菌培养基、酵母菌培养基和显色培养基, 从质控菌株生长率、抑菌效果等方面进行质量分析比对试验。生长率质控标准菌株在各种霉菌培养基、酵母菌培养基上的生长率均值均大于0.5, 在显色培养基上的生长率均值均大于0.7, 符合ISO国际标准。经统计分析, 试验的4家培养基厂家生产的培养基质量水平差异不显著(P > 0.05), 这为将新出台的培养基国家标准拟等效采用培养基ISO国际标准提供了基础材料和依据。

关键词:培养基, 生长率, 抑菌效果, 质量, 食品卫生

Abstract:

To explore the equivalence of ISO culture media standards for domestic culture media quality control, fungus culture and chromogenic media were chosen from four domestic and foreign manufacturers, compared in quality control strain productivity ratio and bacteriostatic effect. The productivity ratioes of productivity reference stains on fungus culture are all above 0.5, and that on chromogenic media are all above 0.7, which comply with ISO international standards. After statistical analysis of these results, there are no significant difference among these culture media (P>0.05) chosen from the four culture media manufacturers in this study, which provide basic materials and basis for new national standard that is about to published to equivalent adopting ISO culture media standards.

Key words:Culture media, Productivity ratio, Bacteriostatic effect, Quality, Food hygiene