铁皮石斛内生真菌分布

distribution of endophytic fungi in Dendrobium officinale

摘要:

本研究从铁皮石斛(Dendrobium officinale)根、茎和叶中分离得到内生真菌67株, 经形态学和分子生物学鉴定分别归属于16个属, Fusarium和Alternaria为铁皮石斛内生真菌的优势种群。研究发现, 铁皮石斛根、茎、叶中内生真菌分布存在较大差别, 具有一定的组织差异性, 不同石斛植物中内生真菌的存在情况差别较大, 具有一定的宿主差异性。本研究丰富了真菌资源, 为石斛属药用植物内生真菌资源的开发利用提供了基础理论依据。

关键词:铁皮石斛, 内生真菌, 鉴定, 分布

Abstract:

A total of 67 strains of endophytic fungi were isolated from roots, branches and leaves in Dendrobium officinale. These strains were identified into 16 genera based on morphological characters and ribosomal DNA(rDNA) sequence analysis, thereinto Fusarium and Alternaria are dominant populations in D. officinale. From analysis results we found some endophytic fungi show a certain degree of host and tissue preference. The research provided theoretical basis for developing and utilizing the resources of endophytic fungi in Dendrobium.

Key words:Dendrobium officinale, endophytic fungi, identification, distribution