以淀粉为碳源衣康酸高产菌株的筛选

Screening of High Itaconic Acid Yielding Strain Using Corn Starch

摘要:

对土曲霉出发菌株进行紫外线诱变、LiCl诱变以及代谢终产物抗性菌株选育。代谢终产物抗性菌株选育是一种有效的遗传育种方法, 能显著提高产酸量。得到一株代号为At394的菌株, 以玉米淀粉部分水解糖为碳源, 产酸量为53.9 g/L, 比出发菌株提高了42.6%。糖酸转化率为61.5%, 为所有筛选菌株最高。用红外光谱进行结构分析证实所得产物为衣康酸。

关键词:衣康酸, 土曲霉, 玉米淀粉, 筛选

Abstract:

This paper described the screening of itaconic acid strain of Aspergillus terreus with UV-irradiation, LiCl treatment and breeding resistant strain to metabolic end product. Breeding resistant strain was an effective measure to help raise yields steadily. A strain, designated At394, producing itaconic acid with a high yield was successfully obtained. The itaconic acid concentration produced by At394 was 53.9 g/L using starch hydrolysate in shaking flasks, which was 42.6% higher than that of the parental strain. The conversion rate was 61.5%, which was the highest value found in tests. The structure determination by infrared spectrum showed the production obtained was itaconic acid.

Key words:Itaconic acid, Asperigillus terrus, Corn starch, Screening