氯仿处理厌氧污泥发酵制氢中微生物多样性的解析

Microbial Diversity Analysis of Anaerobic Sludge with Chloroform Treatment for Fermentive H2 Production

摘要:

研究了不同浓度氯仿对厌氧污泥产氢及其微生物多样性的影响。在氯仿浓度为0.050%时, 累积氢、氢气产率、VFA和总糖降解率均达到最大, 分别为639 mL、1.71 mol H2/mol消耗葡萄糖、2880 mg/L和85%。利用PCR-DGGE技术对不同浓度氯仿处理的污泥样品中微生物多样性和种群结构进行分析显示, 4个细菌克隆属于Clostridia, 2个细菌克隆分别属于Acidobacteria和δ-proteobacteria, 其他4个均属于不可培养细菌。Clostridia中的4个菌群均属产氢菌群, 条带7中含有的细菌可能属于HPB。发酵产氢后的污泥样品C3 (氯仿浓度 = 0.05%)中细菌类型主要有: Megasphaera sueciensis、Megasphaera paucivorans、Clostridium cellulosi、Clostridium sp.和不可培养细菌, 为最适产氢群落结构。

关键词:厌氧产氢, 氯仿处理, PCR-DGGE, 产氢菌

Abstract:

The effects of different CHCl3 concentrations treatment on fermentative H2 production and microbial diversity of anaerobic sludge were investigated. At CHCl3 concentration of 0.050%, the cumulative H2, maximum H2 yield, VFA concentration and total sugar degradability all reached the maximum and were 639 mL, 1.71 mol H2/mol consumed glucose, 2880 mg/L and 85%, respectively. The microbial diversity and community structure of sludge samples with different CHCl3 concentrations treatment were analyzed by applying PCR-DGGE. The results showed that 4 bacteria clones belonged to Clostridia, 2 bacteria clones belonged to Acidobacteria and δ-proteobacteria, respectively, and other 4 bacteria clones were uncultured bacterium. All 4 microbes that belonged to Clostridia were H2-producing bacteria (HPB) and bacteria of band 7 might belong to HPB. The optimum microbial community structure for H2 production was observed in sample C3, which mainly contained Megasphaera sueciensis, Megasphaera paucivorans, Clostridium cellulosi, Clostridium sp. and uncultured bacterium.

Key words:Anaerobic H2 production, CHCl3 treatment, PCR-DGGE, H2-producing bacteria (HPB)