沼泽红假单胞菌生物合成银纳米粒子及其抗菌作用

Biosynthesis of Silver Nanoparticles Using the Phototrophic Bacteria Rhodopseudomonas palustris and Its Antimicrobial Activity Against Escherichia coli and Staphylococcus aureus

摘要:

近年来, 利用沼泽红假单胞菌合成银纳米粒子作为一种可靠和环境友好的方法出现。主要利用沼泽红假单胞菌的细胞滤液来还原银离子。制备的纳米粒子用紫外可见光谱(UV-vis)、X射线衍射光谱(XRD)和透射电镜(TEM)进行表征。含有银粒子溶液的UV-vis光谱显示在420 nm?460 nm处出现银纳米粒子的吸收峰。TEM图像表明所形成的银纳米粒子的粒径范围为5 nm?20 nm。纳米粒子的XRD图谱证明产物为金属银。所制备的银纳米粒子对大肠杆菌和金黄色葡萄球菌作抑菌性试验。

关键词:沼泽红假单胞菌, 生物合成, 表征, 银纳米粒子, 抗菌活性

Abstract:

The use of Rhodopseudomonas palustris in biosynthesis of silver nanoparticles (AgNPs) emerges as a reliable and eco-friendly approach in recent years. This report focuses on extracellular biosynthesis of AgNPs using cell filtrate of Rhodopseudomonas palustris. These nanoparticles were characterized by UV-vis spectrum, X-ray diffraction (XRD) spectrum and transmission electron microscopy (TEM). UV-vis spectrum of the aqueous medium containing silver ion showed a peak between 420 nm?460 nm corresponding to the plasmon absorbance of AgNPs. TEM micrograph showed formation of the AgNPs in the range of 5 nm?20 nm. XRD of the nanoparticles confirmed the formation of metallic silver. The AgNPs were evaluated for their antimicrobial activities against Escherichia coli and Staphyloccocus aureus.

Key words:Rhodopseudomonas palustris, Biosynthesis, Characteration, Silver nanoparticles, Antimicrobial activities