产氢产乙酸菌ZR-1 的分离鉴定及产酸特性

Separation and identification of a hydrogen-producing acetogens strain ZR-1 and its acetic production characters

摘要:

采用改良的亨盖特厌氧操作技术, 从有机废水污泥中分离到一株耐低温高效产氢产乙酸菌ZR-1。经过对其形态学观察、生理生化特征研究及16S rRNA 序列比对, 初步鉴定为梭状芽胞杆菌属的乙二醇梭菌(Clostridium glycolicum)。通过单因子实验, 在厌氧条件下对该菌株的培养温度、pH、最适底物、金属离子的影响等产酸条件进行了优化。结果表明该菌株最适生长温度37 °C,最佳培养基初始pH 值8.5, 最适发酵底物丁酸盐, Mn²⁺对其产酸有一定的激活作用。最适培养条件下丁酸盐降解率达到12.7%, H₂ 含量达到了28.73%。

关键词:厌氧消化, 产氢产乙酸菌, 鉴定, 生酸特性

Abstract:

A hydrogen-producing acetogen strain ZR-1 was isolated from organic wastewater sludge by applying modified Hungate anaerobic method. According to morphological observation, physiologicalbiochemical characteristics and homology analysis of 16S rRNA gene sequence, the strain was identified as a genus of Clostridium glycolicum. The effects of temperature, pH, optimum substrate and metal ions on its productivity of acetic acid in anaerobic conditions were investigated and then optimized by uniform tests. The results indicated that the optimun temperature and initial pH of culture media for strain ZR-1 were 37 °C, 8.5, and it′s optimum substrate was butyrate. Supply of Mn²⁺ in culture medium can promote acetic acid production of ZR-1. At optimum cultivation conditions, the conversion rate of butyrate reached 12.7% and the content of H₂ was up to 28.73%.

Key words:Anaerobic digestion, Hydrogen-producing acetogen, Identify, Acetic acid production characteristics