一株抗香蕉枯萎病内生细菌的分离鉴定及其抗病促生作用

A bacterial endophyte from banana: its isolation, identification, activity to Fusarium Wilt and PGPR effect to banana seedlings

摘要:

从巴西蕉中分离到一株能够明显抑制尖孢镰刀菌古巴专化型4 号生理小种(FOC4)的内生细菌DB09208, 通过生理生化、形态特征及16S rDNA 序列同源性比较, 初步认为它和类芽孢杆菌(Paenibacillus)比较接近。该菌株能够显著抑制FOC4 菌丝生长, 抑菌率为74.09%。盆栽试验表明,该菌株能够抑制香蕉枯萎病的发生, 防治效果达为65.2%, 该菌同时能显著促进香蕉苗生根, 促生效果与植物生长素IAA 相似, 显示出良好的开发前景。

关键词:香蕉枯萎病, 内生细菌, 16S rDNA, 分离鉴定

Abstract:

Strain DB09208, an endophytic bacterium exhibited strong inhibitory activity in vitro against Fusarium oxysporum f. sp. cubense FS-0704 (race 4: FOC4) was isolated from Musa AAA, cv. Baxi grown in Hainan. Based on the morphological, physiological and biochemical properties, and the 16S rDNA sequence analysis, this strain was identified as Paenibacillus sp.. The strain DB09208 obviously inhibited the mycelial growth of FS-0704, with an inhibitory rate of 74.09%, pot experiments indicated the strain suppressed Fusarium wilt of bananas, the control efficiency reached 65.2%. It could significantly promoted the generation of roots with the effect was similar to that of IAA. These demonstrated that strain DB09208 should be useful in biological control bananas Fusarium wilt.

Key words:Fusarium wilt, Endophytic bacteria, 16S rDNA, Isolation and identification