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口蹄疫病毒O/CHN/Mya98/33-P株前导蛋白对病毒感染性的影响
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中国农业科学院基本科研业务费预算增量项目(No. 2013ZL036)


Foot-and-mouth disease virus O/CHN/Mya98/33-P strains leading protein effects on the virus infection
Author:
  • ZHANG Meng

    ZHANG Meng

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • BAI Xing-Wen

    BAI Xing-Wen

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • LI Ping-Hua

    LI Ping-Hua

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • FAN Peng-Ju

    FAN Peng-Ju

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • BAO Hui-Fang

    BAO Hui-Fang

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • SUN Pu

    SUN Pu

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • LU Zeng-Jun

    LU Zeng-Jun

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • CAO Yi-Mei

    CAO Yi-Mei

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • CHEN Ying-Li

    CHEN Ying-Li

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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  • LIU Zai-Xin

    LIU Zai-Xin

    State Key Laboratory of Veterinary Etiological Biology, National Foot-and-Mouth Disease Reference Laboratory, Engineering Research Center of Biological Detection of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu 730046, China
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    摘要:

    【目的】研究口蹄疫病毒(Foot-and-mouth disease virus,FMDV)前导蛋白对于病毒感染性的影响。【方法】利用反向遗传操作技术,以O/CHN/93现用疫苗株的感染性克隆为骨架,分别构建了含口蹄疫病毒中国分离株O/CHN/Mya98/33-P和O/CHN/Mya98/HN1前导蛋白的两株嵌合全长cDNA感染性克隆,采用实时荧光定量PCR检测两株不同的嵌合病毒感染猪肾原代细胞后细胞内IFNβ和OAS mRNA的转录情况。【结果】嵌合病毒rOHN1Lab增殖能力较弱,其对应诱导产生的IFNβ和OAS mRNA含量较高,而嵌合毒rO33Lab相对于rOHN1Lab增殖能力较强,他们诱导产生的IFNβ和OAS mRNA含量较低。【结论】研究表明口蹄疫病毒中国分离株O/CHN/Mya98/33-P前导蛋白具有更强的抗IFNβ mRNA转录的能力,该研究能够为进一步鉴定FMDV前导蛋白抗宿主先天性免疫的关键性位点奠定基础。

    Abstract:

    [Objective] This study is to research the influence of foot-and-mouth disease virus (FMDV) leader protein for virus infectivity. [Methods] Two full-length infected cDNA clones were constructed based on O/HN/93 strain cDNA using reverse genetic technique which contain the leader protein of Chinese isolates O/CHN/Mya98/33-P and O/CHN/Mya98/HN1. Though real-time fluorescent quantitative PCR detecting the situation of IFNβ and OAS mRNA transcription, when pig kidney primary cells were infected the two different chimeric viruses. [Results] We found that the proliferation ability of chimeric virus rOHN1Lab is weaker, and the content of IFNβ and OAS it induced is higher. However, the proliferation ability of chimeric virus rO33Lab relative to rOHN1Lab is higher, and the content of IFNβ and OAS which induced is lower. [Conclusion] This study suggests that the leader protein of the Foot and mouth disease Chinese isolates O/CHN/Mya98/33-P have the stronger ability to resist IFNβ mRNA transcription, which lay the foundation for further identification of FMDV leader protein critical sites for resisting host innate immunity.

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张萌,白兴文,李平花,范朋举,包慧芳,孙普,卢曾军,曹轶梅,陈应理,刘在新. 口蹄疫病毒O/CHN/Mya98/33-P株前导蛋白对病毒感染性的影响[J]. 微生物学通报, 2014, 41(10): 2052-2060

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  • 在线发布日期: 2014-10-10
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