毕赤酵母基因编辑技术研究进展

Advances in gene editing of Pichia pastoris

摘要:

巴斯德毕赤酵母是一种重要的蛋白表达系统，基因编辑技术作为代谢工程的基本工具，对于毕赤酵母的代谢改造十分重要。近十年基因编辑技术发展迅速，除传统的同源重组和Cre/loxP重组外，相继出现了许多新的基因编辑技术，例如ZFN、TALEN和CRISPR/Cas9等，这些技术的出现使基因编辑更加简便高效。本文对毕赤酵母中传统和新型基因编辑技术的原理应用和研究进展进行了简要综述，并结合相关领域的发展对毕赤酵母基因编辑技术的发展进行了展望。

关键词:巴斯德毕赤酵母，基因编辑，同源重组，CRISPR/Cas9

Abstract:

Pichia pastoris is a widely used host for recombinant protein expression. As a basic tool for metabolic engineering, gene editing technology exhibits great importance for the metabolic modification of Pichia pastoris. In the past decade, gene editing technologies have developed rapidly. Many new techniques, such as ZFN, TALEN and CRISPR/Cas9, were developed besides homologous recombination and Cre/loxP recombination which makes gene editing in P. pastoris more convenient and efficient. This review summarized the principle, application and research progress of gene editing techniques in Pichia pastoris. This paper also prospected the future development of gene editing in P. pastoris based on the advances of related fields.

Key words:Pichia pastoris, Gene editing, Homologous recombination, CRISPR/Cas9