草菇9715液体菌种培养过程的生理变化及培养终点

Physiological changes of Volvariella volvacea 9715 during submerged spawn culture

摘要:

【背景】草菇是最具中国特色的食用菌品种之一，其消费量逐年增加，产业发展潜力巨大。液体菌种应用于草菇栽培是其工厂化生产的发展趋势，目前关于草菇液体菌种的研究主要集中在优化配方和生长条件，有关草菇液体菌种培养过程中菌丝活性的研究较少。【目的】研究草菇液体菌种培养过程中的生理活性变化，并确定其培养终点。【方法】对草菇液体菌种培养过程中菌丝体干重、蛋白含量、培养液pH值、糖度、还原糖含量、酶活性等进行测定与分析。【结果】草菇液体菌种在培养84 h后，菌丝干重增长减缓，pH值、糖度、还原糖含量逐渐减少，纤维素酶和半纤维素酶活性逐渐降低，培养96 h后，菌丝体蛋白质含量呈下降趋势，培养液蛋白含量则呈上升趋势。【结论】草菇9715液体菌种培养终点应控制在84?96 h，研究结果可为9715液体菌种应用于草菇工厂化生产提供重要参考。

关键词:草菇，液体菌种，生理变化，培养终点

Abstract:

[Background] Straw mushroom (Volvariella volvacea) is one of the most varieties of edible fungi with Chinese characteristics, and its consumption increased year by year, the potential industrial development for V. volvacea is tremendous. Liquid spawn used in straw mushroom cultivation is the development trend of factory production, the current research in liquid spawn of straw mushroom are mainly focus on the optimized formula and growth condition, however, less research was done on the mycelial activity during the cultivation process. [Objective] Study the physiological changes of straw mushroom in liquid spawn cultivation, and analyze the cultivation end-point. [Methods] Since cultivation 48 h, collection samples every 12 h, the biomass and protein content of mycelia were measured in 168 h. Moreover, pH value, sugar degree, reducing sugar contents, cellulase, hemicellulase and laccase activities of liquid spawn filtrate were determinated and analyzed in the cultivation process. [Results] The results showed that after 84 h of cultivation, the growth rate of mycelia diminishing and the biomass increased slowly, the pH value, sugar degree and reducing sugar contents reduced gradually, cellulase and hemicellulase activities also declined gradually. After 96 h, the protein content of mycelia declined while protein content of filtrate rised obviously. [Conclusion] The research on physiological changes of 9715 liquid spawn indicated that 84?96 h was the cultivation end-point, and the results can provide important reference value for liquid spawn application in the straw mushroom 9715 factory production.

Key words:Volvariella volvacea, Liquid spawn, Physiological changes, Cultivation end-point