Identification of a Vibrio splendidus strain isolated from diseased Sciaenops ocellatus

Abstract:

Abstract: [Objective] Bacterial strain named M-1 was isolated from the diseased Sciaenops ocellatus with ulceration, to further analyze the phylogenetics of the bacterial strain. [Methods] We adopted the characteristics of morphology and physiology, accompanied with the sequence comparison of 16S rRNA and HSP60 genes. [Results] Strain M-1 was identified as Vibrio splendidus. 16SrRNA gene of strain M-1 and Vibrio splendidus(AJ874361) had nearest kinship, and the analysis of HSP60 gene sequence indicated that strain M-1 togethered with Vibrio (EU653883、AY837440)in phylogenetic tree. [Conclusion] The results suggested that strain M-1 was a member of Vibrio splendidus.

Key words:Keywords: Strain M-1; Pathogenicity; 16S rRNA gene; HSP60 gene; Vibrio splendidus