Haihong Han
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, ChinaXiaotao Zeng
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaShengjun Yin
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaRuifu Yang
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaDongsheng Zhou
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, ChinaXiumei Liu
Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100021, ChinaSupported by the National Key Technology R&D program (2006BAK02A15) and; the National Natural Science Foundation of China (30871370); the Important National Science & Technology Specific Projects (2008ZX10004-103)
Abstract：[Objective] Our aim in this study was to develop a whole-genome DNA microarray of Vibrio parahaemolyticus, build up a method of microarray hybridization and carry on microarray quality evaluation. [Methods] Based on the genomic sequences of V. parahaemolyticus, we chose a total number of 4770 genes, amplified them by PCR with specific primers, purified the PCR products and printed them onto glass slides. We performed two sets of hybridizations by the method of two-fluorescence comparative hybridization to evaluate the microarray quality, followed by PCR method to validate parts of microarray results. [Results] Microarray hybridization results were completely consistent with theory expectations and PCR verification results. [Conclusion] We successfully developed a batch of good quality whole-genome DNA microarrays of V. parahaemolyticus, built up a method of microarray-based comparative genomic hybridization of V. parahaemolyticus and a set of microarray data analysis standard method.
Haihong Han, Xiaotao Zeng, Shengjun Yin, Ruifu Yang, Dongsheng Zhou, Xiumei Liu. Development of whole-genome DNA microarray of Vibrio parahaemolyticus and evaluation of the microarray quality. [J]. Acta Microbiologica Sinica, 2010, 50(4): 548-553
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